Signalling

Part:BBa_K1180002:Design

Designed by: Gong Fei ，Wang Wei， Wei Xiuqing   Group: iGEM13_NJU_China   (2013-09-24)


fusion protein of lamp 2b and RVG for brain targeting of the exosome


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1135
    Illegal SpeI site found at 448
    Illegal PstI site found at 832
    Illegal PstI site found at 1218
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 448
    Illegal PstI site found at 832
    Illegal PstI site found at 1218
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1617
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1135
    Illegal SpeI site found at 448
    Illegal PstI site found at 832
    Illegal PstI site found at 1218
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1135
    Illegal SpeI site found at 448
    Illegal PstI site found at 832
    Illegal PstI site found at 1218
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1312
    Illegal BsaI.rc site found at 150


Design Notes

We need to make the RVG peptide as short as possible to avoid the immune response which might be induced by the fusion protein.


Source

The sequence is from the protein lamp 2b and RVG peptide.

References